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This regulatory effect of DMF was identified through NRF2-dependent and independent mechanisms with NRF2 binding to ARE motifs and increasing DNA 5hmC of individual gene regulatory regions involved in the TSS pathway, cystine uptake, and GSH generation

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Ratiometric time-course measurements were carried out with initially oxidized or reduced roGFP2 on a BMG POLARstar Omega fluorescence plate reader with a filter-based excitation at 39010 and 48010 nm

They found that XCHD prevents liver cirrhosis by regulating several key pathways such as the TNF, IL-17, and PI3K-Akt signaling pathway